bstui (New England Biolabs)
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New England Biolabs
bstui
Bstui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bstui/BstU+I/pm41992330-101-90-91
Average 96 stars, based on 1144 article reviews
Bstui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bstui/BstU+I/pm41992330-101-90-91
Average 96 stars, based on 1144 article reviews
bstui - by Bioz Stars,
2026-09
96/100 stars
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Polymerase Chain Reaction:Article Title: Breeding of a Multifoliolate Alfalfa Population Using CRISPR/Cas9-Generated Mutants and Evaluation of Agronomic Traits and Nutritive Value. Article Snippet: .. Amplicons (~768 bp) were gel-purified and primarily analyzed with a PCR-restriction enzyme (PCR-RE) assay using Article Title: Rbm25 governs embryonic stem cell identity and fate through transcriptional regulation of pluripotency and epigenetic programs. Article Snippet: .. For COBRA, ∼200 ng PCR products were digested by TaqI (Takara, 1189A), Article Title: Breeding of a Multifoliolate Alfalfa Population Using CRISPR/Cas9-Generated Mutants and Evaluation of Agronomic Traits and Nutritive Value Article Snippet: .. Amplicons (~768 bp) were gel-purified and primarily analyzed with a PCR-restriction enzyme (PCR-RE) assay using Article Title: The effect of interleukin-21 on HBV-related hepatocellular carcinoma, from the perspective of genetics. Article Snippet: .. The PCR cycling conditions were: initial denaturation at 95°C for 5 min; followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 58°C (for rs12508721 and rs2221903) or 60°C (for rs907715) for 30 s, and extension at 72°C for 30 s; with a final extension at 72°C for 7 min. PCR products were then digested with appropriate restriction enzymes: rs12508721: Digested with BsmAI (New England Biolabs, Ipswich, MA, USA) at 55°C for 4 h; rs2221903: Digested with HinfI (NEB) at 37°C for 4 h; rs907715: Digested with Article Title: Rbm25 governs embryonic stem cell identity and fate through transcriptional regulation of pluripotency and epigenetic programs Article Snippet: .. For COBRA, ∼200 ng PCR products were digested by TaqI (Takara, 1189A), Combined Bisulfite Restriction Analysis Assay:Article Title: Rbm25 governs embryonic stem cell identity and fate through transcriptional regulation of pluripotency and epigenetic programs. Article Snippet: .. For COBRA, ∼200 ng PCR products were digested by TaqI (Takara, 1189A), Article Title: Rbm25 governs embryonic stem cell identity and fate through transcriptional regulation of pluripotency and epigenetic programs Article Snippet: .. For COBRA, ∼200 ng PCR products were digested by TaqI (Takara, 1189A), Agarose Gel Electrophoresis:Article Title: Rbm25 governs embryonic stem cell identity and fate through transcriptional regulation of pluripotency and epigenetic programs. Article Snippet: .. For COBRA, ∼200 ng PCR products were digested by TaqI (Takara, 1189A), Article Title: Rbm25 governs embryonic stem cell identity and fate through transcriptional regulation of pluripotency and epigenetic programs Article Snippet: .. For COBRA, ∼200 ng PCR products were digested by TaqI (Takara, 1189A), Electrophoresis:Article Title: The effect of interleukin-21 on HBV-related hepatocellular carcinoma, from the perspective of genetics. Article Snippet: .. The PCR cycling conditions were: initial denaturation at 95°C for 5 min; followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 58°C (for rs12508721 and rs2221903) or 60°C (for rs907715) for 30 s, and extension at 72°C for 30 s; with a final extension at 72°C for 7 min. PCR products were then digested with appropriate restriction enzymes: rs12508721: Digested with BsmAI (New England Biolabs, Ipswich, MA, USA) at 55°C for 4 h; rs2221903: Digested with HinfI (NEB) at 37°C for 4 h; rs907715: Digested with Staining:Article Title: The effect of interleukin-21 on HBV-related hepatocellular carcinoma, from the perspective of genetics. Article Snippet: .. The PCR cycling conditions were: initial denaturation at 95°C for 5 min; followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 58°C (for rs12508721 and rs2221903) or 60°C (for rs907715) for 30 s, and extension at 72°C for 30 s; with a final extension at 72°C for 7 min. PCR products were then digested with appropriate restriction enzymes: rs12508721: Digested with BsmAI (New England Biolabs, Ipswich, MA, USA) at 55°C for 4 h; rs2221903: Digested with HinfI (NEB) at 37°C for 4 h; rs907715: Digested with Amplification:Article Title: RECAP-seq: restriction enzyme-based CpG-methylated fragment amplification for early cancer detection Article Snippet: 2X KAPA master mix was prepared with KAPA HiFi HotStart PCR Kit (KAPA Biosystems) following the manufacturer’s instructions. .. Amplification was performed in a thermal cycler under the following conditions: initial denaturation at 98 °C for 3 min; 10 cycles of 98 °C for 30 s, 60 °C for 30 s, and 72 °C for 1 min; and a final extension at 72 °C for 1 min. 1,000 ng of amplified EM-seq library was subjected to enzymatic digestion with 0.5 μL of |